Document Details
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Document Type |
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Thesis |
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Document Title |
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Potential Combined Therapeutic Effect of Vitamin D3 and Methotrexate in an Experimental Model of Collagen-Induced Rheumatoid Arthritis التأثير العلاجي المشترك لفيتامين د٣ و الميثوتريكسات في نموذج تجريبي لإلتهاب المفاصل الروماتويدي المحدث بالكولاجين |
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Subject |
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Faculty of medicine |
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Document Language |
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Arabic |
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Abstract |
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Rheumatoid arthritis (RA) is a chronic systemic autoimmune inflammatory disorder that is associated with decreased quality of life. Methotrexate (MTX) is a cornerstone disease-modifying anti-rheumatic drug. Unfortunately, using MTX alone may not fully manage RA and may cause undesirable side effects. Vitamin D3 (V.D3) is one of the most related environmental factors influencing the development of RA in which serum levels of V.D3 correlate negatively with RA activity. So, the aim of the present study was to investigate the potential combined effect of V.D3 and MTX in an experimental model of collagen-induced rheumatoid arthritis in rats.
Methods: Thirty-six Wistar rats were divided into (6) groups; 6 rat each. Group I served as control. The other five groups were immunized intradermal with 2 doses of bovine type II collagen emulsion on day 0 and day 7. Group II served as positive control, group III, IV, V and VI were treated with V.D3 (10 μg/rat &20 μg/rat), MTX (2mg/kg) and combined (V.D3 20 μg/rat & MTX), respectively for 30 days. Arthritis score was evaluated twice weekly, blood samples were collected at the end of the study to assess C-reactive protein (CRP), anti-type II collagen antibodies (anti-CII), tumor necrosis factor alpha (TNF-α), interleukin 1-beta (IL-1β), interleukin 6 (IL-6), albumin, malondialdehyde (MDA), glutathione (GSH), aspartate aminotransferase (AST), alanine Aminotransferase (ALT) and calcium level. In addition to histopathology analysis.
Results: The present findings showed that treatment with V.D3 either alone or in combination with MTX significantly decreased arthritis scores as well as the inflammatory markers (CRP, anti-CII, TNF-α, IL-1β, IL-6) with a significant increase in albumin. Moreover, the oxidative stress marker MDA was significantly decreased while GSH increased as compared to the positive control. For the liver function markers (AST and ALT) and calcium level, a significant decreased were observed when compared to the positive control. Furthermore, most of the histopathological changes were improved by either or combined treatment.
Conclusion: Vitamin D3 showed a dose and time-dependent beneficial effect in the management of RA. This could be related to anti-inflammatory and anti-oxidant effect. Combined therapy of V.D3 and MTX have potentiated the anti-arthritic effect than either treatment alone. This may shed the light on the importance of more experimental and clinical studies to establish the role of V.D3 in treating RA.
Keywords: Rheumatoid arthritis, Collagen induced arthritis, Methotrexate, Vitamin D3, Oxidative stress, Inflammation |
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Supervisor |
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Prof. Dr. Mai Abdul-Alim Abdul-Sattar Ahmad |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Co-Supervisor |
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Dr. Ibrahim Mohammed Ayoub Ibrahim |
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Added Date |
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Friday, December 1, 2023 |
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Researchers
| أفراح عوض القرني | Alqarni, Afrah Awadh | Researcher | Master | |
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