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نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Intracellular localization and properties of phosphate-dependent glutaminase in rat mesenteric lymph nodes
Intracellular localization and properties of phosphate-dependent glutaminase in rat mesenteric lymph nodes
 
الموضوع : الكيمياء الحيوية 
لغة الوثيقة : الانجليزية 
المستخلص : Phosphate-dependent glutaminase was present at approximately similar activities in lymph nodes from mammals other than rat, and in thymus, spleen, Peyer's patches and bone marrow of the rat. This suggests that glutamine is important in all lymphoid tissues. Phosphate-dependent glutaminase activity was shown to be present primarily in the mitochondria of rat mesenteric lymph nodes, and most of the activity could be released by detergents. The properties of the enzyme in mitochondrial extracts were investigated. The pH optimum was 8.6 and the Km for glutamine was 2.0 mM. The enzyme was activated by phosphate, other phosphorylated compounds including phosphoenolpyruvate, and also leucine: 50% activation occurred at 5, 0.2 and 0.6 mM for phosphate, phosphoenolpyruvate and leucine respectively. The enzyme was inhibited by glutamate, 2-oxoglutarate, citrate and ammonia, and by N-ethylmaleimide and diazo-5-oxo-L-norleucine; 50% inhibition was observed at 0.7 and 0.1 mM for glutamate and 2-oxoglutarate respectively. Some of these properties may be important in the control of the enzyme activity in vivo 
ردمد : 0264-6021 
اسم الدورية : The Biochemical Journal 
المجلد : 217 
العدد : 1 
سنة النشر : 1404 هـ
1984 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Monday, November 28, 2011 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
محمد العرضاويArdawi, Mohammed باحث رئيسيدكتوراه 

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اسم الملفالنوعالوصف
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