تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Successful propagation of Alkhumra (misnamed as Alkhurma) virus in C6/36 mosquito cells
Successful propagation of Alkhumra (misnamed as Alkhurma) virus in C6/36 mosquito cells
 
الموضوع : الأمراض المعدية 
لغة الوثيقة : الانجليزية 
المستخلص : Epidemiological data suggest that Alkhumra (misnamed as Alkhurma) virus (ALKV) is transmitted from livestock animals to humans by direct contact with animals or by the mosquito bites, but not by ticks. To assess the ability of the virus to replicate in mosquito cells, serum and plasma of seven acutely febrile patients with clinically suspected ALKV infection reported in Najran, Saudi Arabia in 2009 were inoculated onto Aedes albopictus mosquito cells (C6/36) and directly examined with ALKV-RNA-specific real time RT-PCR as well as indirect immunfluorescence assay (IFA) using ALKV-specific polyclonal antibodies. The isolated virus was titrated in the mammalian rhesus monkey kidney cells (LLC-MK2). Five of the seven specimens were RT-PCR- and culture-positive demonstrating cytopathic effects in the form of cell rounding and aggregation appearing on day 3 post inoculation with syncytia eventually appearing on day 8 post inoculation. Identification of ALKV-RNA in the cell culture was confirmed with RT-PCR and IFA. The virus titre was 3.2×10(6) tissue culture infective dose 50 (TCID(50)) per mL. Three more viral passages were successfully made in the C6/36 cells. This is the first description of propagation of ALKV in mosquito cells 
ردمد : 0035-9203 
اسم الدورية : Transactions of the Royal Society of Tropical Medicine and Hygiene 
المجلد : 1 
العدد : 1 
سنة النشر : 1433 هـ
2012 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Thursday, January 5, 2012 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
طارق مدنيMadani, Tariq باحث رئيسيدكتوراه 
حسين البارAl-Bar, Hussein باحث مشاركدكتوراه 

الملفات

اسم الملفالنوعالوصف
 31984.pdf pdf 

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