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Document Type |
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Article In Journal |
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Document Title |
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BACTERICIDAL ACTIVITY OF LEVOFLOXACIN ALONE AND INCOMBINATION WITH CLINDAMYCIN OR N-ACETYL CYSTEINE AGAINST BIOFILM PSEUDOMONAS AER UGINOSA AND STAPHYLOCOCCUS A UREUS BACTERICIDAL ACTIVITY OF LEVOFLOXACIN ALONE AND INCOMBINATION WITH CLINDAMYCIN OR N-ACETYL CYSTEINE AGAINST BIOFILM PSEUDOMONAS AER UGINOSA AND STAPHYLOCOCCUS A UREUS |
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Document Language |
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English |
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Abstract |
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Bactericidal activity of levofloxacin alone and in combination with clindamycin (20 µg/ml) or N-acerylcysteine (1 mg/ml) was studied against clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus (10 isolates each) in suspensions and in biofilm (formed by overnight incubation of 105-106 CFU/ml in the wells of microtiter plates). Viable counts of all the sets were done on Mueller-Hinton agar plates at 0, 2, 4, 6, 12, 24 hr periods. The presence of levofloxacin at concentration equivalent to MBC and 2MBC (2-8 times MIC) reduced the counts (log CFU/ml) of P. aeruginosa, grown in suspension, from 6.7-8.2 at 0 hr to 3.6-4.4 and 1.7-2.8 at 24 hr, respectively, and of S. aureus grown in suspension, from 6.8-8.2 at 0 hr to 3.6-4. 7 and 3.1-4.1 at 24 hr, respectively (control = 8.0-8. 7). The presence of clindamycin or N-aceiylcycteine did not significantly increase the bactericidal activity of levoflloxacin against P. aeruginosa isolates in suspension. When these isolates were grown as a biofilm, the bactericidal activity of levofloxacin at concentration of 1 MBC was significantly reduced (P < 0.01) as compared to that obtained against the tested organism in suspension, while at concentration of 2 MBC no significant change in the activity was observed (P < 0.01). In the presence of clindamycin or Nacetylcysteine, the activity of levofloxacin against the biofilm P. aeruginosa was significantly increased (P < 0.01). In case of S. aureus the presence of Nacetylcysteine did not significantly increased the bactericidal activity of levofloxacin against the organism in suspension form, but significantly increased against biofilm associated bacteria. These data suggest that the presence of clindamycin and Nacetylcysteine enhanced the bactericidal activity of levofloxacin against biofilm associated P. aeruginosa and S. aureus. |
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ISSN |
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3185-4152 |
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Journal Name |
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Egypt. J. Biomed. Sci. |
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Volume |
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19 |
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Issue Number |
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6 |
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Publishing Year |
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1425 AH
2005 AD |
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Article Type |
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Article |
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Added Date |
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Sunday, May 30, 2010 |
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Researchers
| سليمان الشيخ | EL-sheikh, Soliman | Researcher | Doctorate | |
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