Document Details
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Document Type |
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Article In Journal |
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Document Title |
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EFFECT OF DIFFERENT ANTIMICROBIAL AGENTS ON STAPHYLOCOCCUS A UREUS ADHESIVENESS AND BIOFILMFORMATION EFFECT OF DIFFERENT ANTIMICROBIAL AGENTS ON STAPHYLOCOCCUS A UREUS ADHESIVENESS AND BIOFILMFORMATION |
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Document Language |
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English |
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Abstract |
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ABSTRACT
The effect of fluoroquinolones (ciprofloxacin, ofloxacin, and levofloxacin), clindamycin, β-lactams (cefoperazone, cefotaxime, cefepime) streptomycin, and vancomycin on the adherence and biofilms formation by Staphylococcus aureus (12 clinical isolates) was studied In the presence of 1/2 MIC, 1/4 MIC and 1/8 MIC’, the optical density of the formed biofilm on plastic surfaces was reduced to 24-59. 9%, 32.4-76.7% and 49.7-88.5% of the controls, respectively. Treatment of the preformed biofilm with high concentrations (25-200 µg/ml) of the tested agents caused reduction in the optical density of the adherent biofilms to a range from 52.3 to 87.7% of the control. In an in-vitro model of vascular catheter colonization, the tested sub inhibitory concentrations reduced the percentage of the viable adherent cells to 32.1-71.6%, 42.5-85.6%, and 60.3-95.3% of the controls, respectively. The tested fluoroquinolones and clindamycin are significantly more active than the other tested agents, and levofloxacin was the most active one. The vascular catheter segments precolonized with S. aureus for 24 hr and exposed to 50 µg/ml (4-3 1 times MIC) of the tested fluoroquinolones and clindamycin for 2 hr showed few viable adherent cells (4-311 CFU/segment), while no adherent viable cells were cultured in the presence of 100 µg/ml (8-62 times MIC). Also, the tested sub inhibitory concentrations reduced the percentage of the viable bacterial cells adherent to the surface of human lung epithelial A549 cells to the range of 30.1-79.2%, 41.1-89.3%, and 60.9-96.2% of the control, respectively. Treatment of the A549 cells, pre-attached with bacterial cells. with the tested agents at concentrations of 5, 10, and 20 µg/ml (1/4-50 times MIC) reduced the range of the percentage of the adherent cells to 53.2-88.3%, 33.8-79.2%, and 27.2-68.1% of the control, respectively. The superior activity of the tested fluoroquinolones and clindamycin was also observed. The obtained data show that subinhibitory concentrations of ciprofloxacin, ofloxacin, levofloxacin, and clindamycin efficiently reduced the biofilm formation and adherence of S. aureus to the surfaces of plastics, vascular catheters, and human lung epithelial A549 cells. Also, higher concentrations (> MIC) offluoroquinolones and clindamycin were able to eradicate the adherent S. aureus. |
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ISSN |
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1547190 |
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Journal Name |
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N. Egypt. J. Microbiol. |
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Volume |
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13 |
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Issue Number |
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2 |
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Publishing Year |
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1426 AH
2006 AD |
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Article Type |
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Article |
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Added Date |
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Sunday, May 30, 2010 |
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Researchers
| سليمان الشيخ | EL-sheikh, Soliman | Researcher | Doctorate | |
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