Document Details

Document Type : Article In Journal 
Document Title :
Fatty acid profiles of sebaceous triglycerides by capillary gas chromatography with mass-selective detection
Fatty acid profiles of sebaceous triglycerides by capillary gas chromatography with mass-selective detection
 
Subject : Biochemistry 
Document Language : English 
Abstract : Fatty acid methyl esters prepared from the triglyceride fraction of skin surface lipids from six adult human males were chromatographed on a 50-m fused-silica column coated with the highly polar cyanopropylpolysiloxane phase. This permitted the resolution of double-bond positional and geometric isomers. By means of mass-selective detection, 33 saturated and 14 unsaturated fatty acid chain types were analysed. Interpretation of the mass spectra combined with precise calculation of equivalent chain length values permitted the identification of 22 saturated and all of the unsaturated chain types. Quantification by integration of total-ion and selected-ion chromatograms revealed marked variation in the triglyceride fatty acid composition between different subjects. The greatest variation was observed in the concentrations of even-carbon-numbered iso-branched acids, which ranged from 1.5 to 11% of the saturated and from 1.9 to 12.7% of the monounsaturated acids. The anteiso chain structures constituted 4-9% of the saturated and 3-6% of the unsaturated members. Fatty acids with 4-methyl branch showed the least variation, in the range 5.7-7.4%. Other methyl-branched acids made up 4-10% of the saturated group, but were not detected in the unsaturated acids fraction. Two 18:1 fatty acids were identified (a delta 8 and a delta 9), which possibly have different anatomical origins. Similarly, two 18:2 fatty acids (linoleic and a 2,3-dimethyl derivative) were identified. A 2-methyl C17 acid, probably of bacterial origin, was detected 
ISSN : 0021-9673 
Journal Name : Journal of Chromatography 
Volume : 425 
Issue Number : 1 
Publishing Year : 1408 AH
1988 AD 
Article Type : Article 
Added Date : Saturday, December 3, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
زهير المرزوقيMarzouki, Zohair InvestigatorDoctorate 

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